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He compared Philographics to his time in grade school attempting to digest whole subjects, some several hundred pages long, into only several tight pages, relying solely on key words and images to study for exams.
EcoRI was used to partially digest whole genomic DNA and the resulting fragments were cloned in pBACe3.6 as described previously [18].
As Devin Faraci pointed out on his site Badass Digest, whole chunks of storyline that Sony used while marketing "The Amazing Spider-Man" were not present in the actual finished film.
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Marin County does not lend itself to digesting whole.
Previous work in our laboratory has shown that the pulsed-field gel electrophoresis (PFGE) method of XbaI digested whole genomes gave good discrimination and was highly reproducible [ 6].
MSCs were cultured from all individual cord regions, as well as enzymatically digested whole cord, demonstrated by their plastic adherence, flow cytometry profile, and ability to differentiate along osteogenic, adipogenic, and chondrogenic lineages.
All of the objects that had been subjected to PGAA/PGAI at Budapest were subsequently digested whole in 5 M nitric acid (HNO3), according to previously published protocols (Rimmer 2010; Rimmer et al. 2012).
We first obtained a deep skeletal muscle proteome by processing and enzymatically digesting whole muscle homogenates by the filter-aided sample preparation (FASP) method and separating the resulting peptide mixture into 12 fractions by isoelectric focusing [ 16, 17].
The overall genetic background of the isolates was evaluated: 1) by digesting whole cell DNA with SmaI macrorestriction enzyme and determining the fragment-size patterns obtained on pulsed-field gel electrophoresis (PFGE) using a contour-clamped homogeneous electric field system on a CHEF DR-II apparatus (Bio-Rad Laboratories, Marnes-la-Coquette, Frasce) as previously described (19 ).
The film, often doggedly cheerful, will not please uniformly, and only die-hard fans of musicals, very earnest people and Sept. 11 completists are likely to digest it whole.
Identifying the genes for the enzymes that do this, modifying them and assembling them into new pathways would produce systems that could digest the whole plant and turn it into ethanol.
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