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The DNA content was determined from the papain digest using a Picogreen DNA assay (Invitrogen) in accordance with the manufacturer's instructions.
The relative fractions of methylated and unmethylated DNA are subsequently determined by comparing the amount in each digest with that of a mock (no enzymes added) digest using a ΔCT method.
In 1936, the Literary Digest, using a telephone and postcard poll, forecast a sweeping victory for Alfred Landon over Franklin Delano Roosevelt.
Fractionate the digest using a 1% agarose gel, elute DNA in 20 μl ddH2O using Qiagen gel extraction kit.
Cleaved Pikp-HMA was purified from the digest using a Ni2+-NTA column, collecting the eluate, followed by dialysis in buffer B and was then concentrated as appropriate.
The Fab portion was purified from the digest using a Protein A column, after which Fc fragments and remaining whole IgG were eluted.
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Vessels content were mixed and kept at room temperature for 12 h, then the vessels were introduced in the microwave digestion system and digested using a four-step digestion program: (1) 5 min at 280 W, (2) 5 min at 700 W, (3) 10 min at 1,050 W, (4) 1 min at 0 W.
Sikor samples originating from Bangladesh were digested using a microwave digester and analysed for their As, Pb, Cd, Mn, Fe and Zn levels using ICP-MS.
300 mg of milled seeds were then digested using a modified protocol of dry ashing digestion (Jiang et al. 2007).
Composite soil samples (0 10 cm depth) were digested using a nitric and hydrochloric extraction technique.
The peel was anaerobically digested using a consortium of microorganisms from cattle rumen content as inoculum.
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