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Conventional injection of a 20 nL sample volume produced a separation window from 10to2525 min. We then performed a dynamic pH junction injection of a 0.05 mg/mL BSA digest prepared in 10 mM ammonium acetate (pH ∼6.5).
They say that because they do not know what is presented in the digest, prepared by the C.I.A.'s intelligence directorate, they have no way of challenging information with which they disagree, administration officials said.
An intelligence digest prepared six mornings a week, by the Central Intelligence Agency, it is one of the most highly classified documents prepared by the government, its distribution limited under President Bush to fewer than a dozen senior administration officials.
The higher amounts of micellarised carotenes (80%) were found in the digest prepared from cooked carrots containing 10% olive oil.
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We therefore analyzed Kac-enriched tryptic digests prepared from pull-downs of salt-extracted histone using C-terminally biotinylated BRD4(1) and BRD4(2) by mass spectroscopy.
In order to maximize the proteome depth for the generation of a SWATH ion library, tryptic digests prepared with the RapidACN protocol were separated by high pH reverse phase chromatography before LC-MS/MS analysis.
Digests (prepared in 0.1% TFA (trifluoroacetic acid)/water) were concentrated on a 20×0.1 mm nanotrap column (Thermo) equilibrated in 0.1% TFA/water (10 μl/min) and washed with 10 μl of the same buffer.
Those of Messrs. Lewis and Clarke, and Freeman, will require further time to be digested and prepared.
Samples were lyophilized and weighed, then digested and prepared as previously described.
For example, libraries can be constructed from pools of DNaseI digested fragments prepared from parental templates [5], [6], [7], [8], from degenerate oligonucleotides [9], [10] or from mixtures of both, or even from undigested parental templates [11], [12], [13], and are usually assembled through PCR.
Protein was digested and prepared for either N-glycan or O-glycan analysis.
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