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This method may be applicable to most antibody digest preparations.
(A) Representative examples of retinal digest preparations.
Vascular cell composition and patency were assessed by quantitative retinal morphometry of digest preparations.
Quantitative retinal morphometry was carried out in retinal digest preparations as described previously [21].
To investigate the response of vasculature with regards to neuronal damages in TGR rats, we assessed the formation of acellular capillaries (ACs), the number of endothelial cells (ECs) and pericytes (PCs) in the retinal vasculature in retinal digest preparations.
Retinal morphology was analyzed by retinal digest preparations and quantitative morphometry in 8-month-old ZDF rats as described [ 8].
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Also other amino acids like asparagine, glutamine, alanine, proline, and leucine were found in higher amounts in dynamically digested preparations.
Evidence for linkage was partially or totally abrogated in the EcoRI digested DNA preparations.
Products ranging in size from 18 to 33 Kb were extracted from the gel (estimated 60 ng DNA total) following the protocol of GELase Agarose Gel-Digesting Preparation kit (Epicentre, Madison, WI 53713), and used to prepare the cosmid library.
The rest of the plugs were melted and digested by agarase according to instructions of the GELase Agarose Gel-Digesting Preparation Kit (Epicentre).
Cryo-electron microscopy was applied to assess particle shape prior to injection, ex vivo fluorescence images of dissected tissues were acquired, excised tissue was further processed with a cell-digest preparation and assayed for fluorescence.
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