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Soybean-casein digest medium was purchased from Becton, Dickinson and Company (Franklin Lakes, NJ, USA).
After a 24-h period for radioactive decay, two 100-μL aliquots were taken from each sample and mixed with soybean casein digest medium and fluid thioglycollate medium, respectively, and incubated for 14 days at 37 °C.
Briefly, cells were obtained by perfusion of mouse liver lobules with liver perfusion and liver digest medium (Gibco) at 37°C.
The tissue was washed with PBS and then placed in collagenase-dispase medium (Liver Digest Medium, Invitrogen) at 37°C for 90 min as we described previously [21].
Liver digest medium (Life Technologies) was added during four consecutive 30-min incubations at 37°C.
S. aureus was grown in soybean-casein digest medium under aerobic conditions at 37 °C until late log phase.
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People can also more easily digest medium-chain triglycerides and convert them to energy, according to The Wall Street Journal, making coconut oil a good choice for athletes.
The digested tissue was then gradually forced through a 100 µm, 70 µm and 50 µm cell strainer to achieve a single cell suspension, and after removal of the digesting medium resuspended in growth medium [IMDM containing 10% fetal bovine serum (Invitrogen), 100 ng/ml EGF, 100 ng/ml bFGF and 10 ng/ml SCF (R&D Systems , Inc Minneapolis, USA)] and plated in flasks.
The pieces were then incubated in digesting medium (1.5% cellulose enzyme [Sigma], 1% Hemicellulase [Sigma], 0.5% pectolyaser [Onazuk, Japan] in 10 M CPW) for 4 h at 30°C in the dark.
Then again as a father and a natural optimist I find it hard to really digest the medium term consequences in relation to our lifestyle and my son's future, so I am likely to reject some of the more extreme implications on our future freedoms.
To enrich for cellular protein and obtain RNA from bone tissue, calvarial bones from four to five day-old pups were dissected from soft tissues, rinsed with HBSS, and digested in collagenase digestion medium [52] on an orbital shaker for 20 minutes at 37 degrees at 125 rpm.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com