GSTs also break down a variety of compounds produced during metabolism and digestion, such as partially digested lipids, in addition to their involvement in the detoxification of environmental toxins, plant allelochemicals, and organochlorine and organophosphate insecticides [ 18- 20].
Altogether, this experiment demonstrates the ability of isolated sperm proteasomes to digest an intact pig zona, in addition to digesting soluble zona proteins.
For a final identification, the amplified 16S rDNA of some strains were digested in addition with BfaI (New England Biolabs, USA; sequence: C^TAG) or HpyF10VI (Fermentas; sequence: GCNNNNN^NNGC).
In addition to digesting the protein with pepsin, digestion with Fungal XIII was performed in solution to increase sequence coverage.
The restriction enzyme BcnI (Fermentas, St Leon-Rot, Leon-Rot, Germany the rs25531 variant digestedthelly, in addition to two constitutive rs25531tion sites in the amplicon.
In addition to digesting naked DNA, the MNase is known to cleave AT/TA dinucleotide motifs as well as showing other, more subtle, sequence specificity (Horz and Altenburger, 1981).
http://dx.doi.org/10.1016/j.coph.2013.08.013 The gastrointestinal (GI) tract, in addition to digesting food and absorbing the available nutrients, releases hormones with important physiological roles in regulating plasma glucose levels, gut motility and satiety.
MALDI TOF MS analysis of exhaustive pronase/aminopeptidase digests of rHSA demonstrated that in addition to lysyl residues, two other amino acyl residues, cysteinyl and histidyl, are involved in adduct formation.
In addition to the sialidase digests, further digests with α-galactosidase, β-galactosidase and β-hexosaminidase confirmed the presence of Gb3, LacCer and Gb4 glycan structures in both samples (data not shown).
In addition to DNA fragments ∅X-174-RF HaeIII digesizingA sizing of hepatitis C viral (HCV) amplicon is also achieved using microchip electrophoresis on PMMA substrates.
