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In addition, grapevine-specific proteome tryptic digest databases were created from both the translation of coding regions found within the Vitis vinifera genome sequence [58], as well as the EST-derived protein set utilized by [12].
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OTUs were identified based on an empirical TRFLP database (Bokulich and Mills, 2012a) and an in silico digest database generated with MiCA (Shyu et al., 2007) of good-quality 16S rRNA gene sequences from RDP (Cole et al., 2014), allowing up to three nucleotide mismatches within 15 bp of the 5′ terminus of the forward primer.
We obtained two BoNT/B2 samples whose sequences were not in the protein database, digested those samples, and the resultant peptides were analyzed by LC-MS/MS.
To test the ability of the method to detect differences within subtypes (<1.4% amino acid variation which is ~21 amino acids), we obtained two BoNT/B1 samples whose amino acid sequences were not in the protein database, digested those proteins, and analyzed the resultant fragments by LC-MS/MS.
The database provides the in silico trysin digest of the proteins and the possible NXS/T motifs.
Zoho Reports, which was formerly known as Zoho DB, provides developers and database administrators with better ways to manage, digest and understand their data.
Zoho DB is meant to provide developers and database administrators with better ways to manage and digest their data.
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