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The constructed recombinants underwent restriction digest analysis with XhoI and the digestion products were subjected to agarose gel electrophoresis; the result showed a clear electrophoretic band at approximately 5.8 kb, consistent with the theoretical results.
In sum, consistent with the ∼25 kDa fragment size, and digest analysis, the main structural domain of myoc-OLF harbors a disulfide bond and is likely inclusive of residues Ser 238 and Lys 461.
Restriction digest analysis of the invading strand in the plasmid D-loops was conducted in reactions (20 µl) containing 50 pM purified D-loops and 10 units of the restriction enzyme indicated in Fig. 1 for 4 5 hr at 37°C, according to the manufacturer's protocol.
Restriction digest analysis of pJGW07.
Restriction digest analysis of amplified exon 1 also confirmed the sequencing data (Supplementary Fig. 1).
Although restriction digest analysis is an efficient technique to analyze editing frequency, the restriction digest analysis does not offer complete information about the nature of the indels present at the EZH2 locus.
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Print sales jumped by 21% week on week on results day on Friday and surged 25% on the Saturday as people digested analysis of the "no" result.
Snap freeze the peptide digest until analysis by matrix assisted laser desorption ionization (MALDI)- time of flight (TOF) mass spectrometry (MS).
A detailed description of the protein digest, peptide analysis with liquid chromatography-tandem mass spectrometry, and database searches is given in the Supplemental Experimental Procedures.
For protein digests analysis with the LTQ, data dependent acquisition was applied.
For separation, 300 V/cm was applied for BSA digests and E. coli digests analysis, and 400 V/cm was applied for intact protein analysis.
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