Probes for Southern and northern analyses were labeled by the PCR-DIG procedure (Roche Molecular Biochemicals).
For every hybridization procedure, DIG-labeled cRNA sense probes were used as control to rule out nonspecific binding.
The famous wax thought experiment of the Second Meditation is supposed to illustrate (among other things) a procedure to "dig out" what is innate.
Description of procedure in digging and laying out a claim.
(2006) expanded a wavelet-based statistical process control (SPC) procedure for dig-up out-of-control events that signal course of action abnormalities for monitoring complicated data.
Forensic accounting is an in-depth procedure that digs beneath the surface of recorded transactions.
Lincoln's procedure was to dig up his specimens, take them to his studio for their portraits and then return them, still living, to their homes.
The RNA was blotted onto a positively charged nylon membrane (Roche) according to standard procedures, and the transcript of interest was detected using DIG-labeled antisense probes (DIG labeling and detection, Roche).
Every year the parents went into debt over Christmas and, literally, it took them a year to dig out, only to repeat the procedure the next year.
The PCR products were purified and used as template for in vitro transcription using the SP6 polymerase in the presence of Digoxigenin-UTP, following the procedure described in the DIG RNA labeling Kit (Roche Diagnostics, Almere).
Southern hybridization was performed following standard procedures [ 78] using the DIG nonradioactive labeling and detection system (Roche, Germany).
