However, technical difficulty to isolate and characterize these progenitor types due to the complex lung structure continues to hinder the understanding of the role of endogenous regenerative pathways in lung tissue maintenance and repair in response to injuries.
The functional heterogeneity among cancer stem cells and bulk cells must reflect distinct cellular epigenetic landscapes, but — due to the difficulty to isolate bona fide cancer stem cells with a high degree of purity — those different epigenetic landscapes, and the molecular mechanisms underlying them, remain largely unknown.
We have not been able to measure intracellular NAD levels in inflammatory cells isolated from the paws of CIA mice due to the paucity of the cells and the difficulty to isolate them with high purity.
Studies on the feasibility of immunotherapeutic strategies in bone tumors are often hampered by the technical difficulty to isolate viable fresh tumor cells for functional testing.
Because of the difficulty to isolate and obtain an uncontaminated population of latently infected cells, only a limited number of studies investigated the cellular transcriptome of HIV latency in primary models.
However, because of the difficulty to isolate the in vivo apical and basal cells of two-celled proembryo from ovule and ovary in higher plants, the transcriptome analysis of them hasn't been reported.
This goal is, however, complex because of the difficulty to isolate, in an intact network system, the contribution of these conductances in the absence of selective inhibitors, the widespread dysfunction of motoneurons and the lack of knockout rat models.
We believe our described model would represent an ideal test bed to monitor implant adjacent bone loss due to these low virulence microorganisms over time, particularly as there is continued controversy regarding the relative contribution of difficulty to isolate microorganisms such as P. acnes in loosening of prosthetic devices [ 35].
Although epidemiological studies show difficulties to isolate the links between the different parameters involved, some results could guide us to relate the effects of Electromagnetic Fields with these kind of diseases.
Facing the safety concerns of ESCs, and the difficulties to isolate and exploit adult stem cells for dental therapies in clinics, induced pluripotent stem cells (iPSCs) which could be another option to regenerate lost tooth or to repair a tooth injury.
