The tested images included many that pose difficulties to existing image analysis tools.
Several factors such as presence of paralogs, small exons, non-consensus splice signals, sequencing errors and polymorphic sites pose recognized difficulties to existing spliced alignment algorithms.
Another strong difficulty to overcome is existing: the strong difference of scales between the wavelength of the focusing laser spot size (currently close to the micrometer range) and the nanostructures sizes (close to the nanometer range).
In addition, more stimulative aggregate demand policies are perceived to result in balance of payments difficulties at existing exchange rates.
Due to computational difficulty, existing methods either handle only small pedigree data or disregard the pedigree structure.
Diseases transmitted by mosquitoes have a devastating impact on global health and this is worsening due to difficulties with existing control measures and climate change.
In AN, both avoidance of treatment [ 12] and dropouts - ranging between 20 and 51% in the inpatient population and from 23 to 73% in outpatient samples – are related to the great difficulty existing in engaging and maintaining their treatment adherence [ 13, 14].
We note that previous comparative studies have highlighted the difficulties of existing programs to assign consistently secondary structure to coordinate data and have proposed using a 'consensus' definition secondary structure assignment that is at the intersection of all the methods to arrive at a reliable simplification of protein structures (Colloc'h et al., 1993; Cuff and Barton, 1999).
Previous comparative studies have highlighted the difficulties of existing programs to assign secondary structure consistently (Andersen and Rost, 2009; Colloc'h et al., 1993; Cuff and Barton, 1999; Martin et al., 2005; Zhang et al., 2008).
On the demand side, we see the following elements: i. Difficulties for existing community groups to raise 60% of the membership or 100 families per village prior to enrolment; ii.
