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Still, no attempt to quantify the contamination level of the pens was performed in the present study, due to an expected uneven distribution of Salmonella in the contaminated pens with a subsequent difficulty to define the true pathogen load.
Finally, it has to be underlined that the focus on CNVRs is accompanied by (a) the difficulty to define the CNV markers that make up a CNV and (b) the difficulty to assess when and if association signals in different samples pertain to the same source.
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However, it is not suitable to model real world physical phenomena because it has difficulties to define the fractional order physical conditions such as initial condition.
This may be due to difficulties to define the correct landmarks on the tibial tubercle during surgery as well as during measurement.
Unfortunately, owing to the low number of patients studied and the difficulties to define the time length from infection to ICU admission, the article by Monserrat and colleagues [ 1] cannot offer a proper analysis of these issues and leaves the question open.
On the other hand, the same paper emphasises the difficulty to define precisely the effects of animal welfare on animal health and food safety.
In light of the aforementioned explanation, there is no difficulty to define PSD in the follow-up coordinates.
The complexity to human rights stems from the level of difficulty to define human rights whereas the failure to make reliable prediction could be due to unavailability of a reliable approach to measure and monitor the human rights [ 18, 19].
Theoretically, the problem persists mainly due to the difficulty to define an appropriate parameter for determining the diameter.
The difficulty to define plagiarism can partly be related to the different attitudes towards it (Helgesson, 2015; Sutherland-Smith, 2008).
A limitation of FBA, MOMA and FVA is the difficulty to define a relevant objective function: for example, the objective function to predict cell growth typically involves a linear combination of more than 100 metabolites (Raman and Chandra, 2009).
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