In spite of the difficulties, the development of an effective genetic transformation protocol is important for engineering a bacterial host for bioproduction, especially to a potential host with unique physiological properties, such as butanol-tolerant bacteria.
Delay of cleft palate treatment significantly beyond the 7- to 18-month time frame may result in difficulty with the development of normal speech, since the brain will mature prior to achieving a functioning palate.
Due to the difficulty of the development's two-and-a-half-acre site, which is on a steep slope rising 100 feet from the Metro-North railroad tracks to Livingston Street, the developer had to design a range of housing configurations to fit the topography.
Valentin Spiess, the CEO of Swiss engineering firm AG, who worked with Khan on the pavilion's facade design, said: "The difficulty was the development of a system that would meet Asif's requirements of the project in relation to speed, usability and image quality.
Literature confirms the difficulty in the development of objective measures for flexibility, for example, because of its property of multidimensionality.
These shortages cause much difficulty in the development of the silicon-based materials as commercial anode materials.
The main difficulty in the development of ATP antagonist kinase inhibitors is target specificity, since the ATP-binding motif is present in many proteins.
For samples of CaCu2.9Ti4O12, the lower dielectric constant can be attributed to the smaller grain boundary areas and the difficulty in the development of domains inside the fine grains.
The difficulty in the development of anticancer medicines is well reflected in the fact that only 5% of the candidates entering clinical trials reach the approval for marketing [1].
An additional difficulty in the development of QSAR models is the fact that some regression methods have parameters that need to be optimised to obtain the best performance for a particular problem.
