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There is also a 1 bp insertion (T) in KW-C ITS1, which probably accounted for the difficulty sequencing the mixed ITS amplicons directly.
These impairments result in inconsistent productions of the same words, difficulty sequencing speech sounds together to form fluent words and sentences and impairments of the melody (i.e. prosody) of speech.
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Despite these difficulties, sequencing whole cancer genomes is clearly a major advance from which we will learn a significant amount about the genes involved in cancer formation and how the cancer genome evolves.
However, 23S rRNA has lost favor to 16S rRNA in phylogenetic analysis and is rarely used in taxonomic classification because of the lack of established broad-range sequencing primers and the difficulty of sequencing larger genes with early sequencing technology.
The ambiguous bases in the ENSEMBL genome sequence may result from the difficulty in sequencing the repetitive region, or perhaps from the polymorphic state of the MITE in the sequence template.
Another limitation of the Sanger method is its difficulty in sequencing GC-rich regions.
33 Another difficulty in sequencing Plasmodium clinical samples is posed by polyclonality, that is, mixed genotype infections.
A few mutations in the sense strand were introduced in all constructs to avoid plasmid instability and difficulty in sequencing.
Because of ease of sequence analysis, DArT markers have a significant advantage over AFLPs for positional cloning efforts due to the difficulty in sequencing AFLPs that, therefore, cannot be readily integrated into the whole genome sequence.
Detecting indels was not as reliable as detecting base substitutions due to difficulty in sequencing homopolymer regions; sequencing accuracy of homopolymers falls to 97 % at homopolymer stretches of five or more base pairs [ 21].
None of these non-SNP polymorphisms was considered for the diversity analysis, partly because of their different mutational nature to SNPs and partly because of the intrinsic difficulty in sequencing through some of these regions.
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