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The largest obstacle facing scientists interested in estimating parameters for wild salmonid populations is the difficulty sampling all (or even most) breeding individuals and their offspring, i.e., difficulty obtaining the samples with which to reconstruct the pedigree (e.g., Dickerson et al. 2005).
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Nevertheless, their measurement is not easy on account of heterogeneity in property and difficulty of sampling.
However, population genetics studies of nematodes have been constrained due to the difficulty in sampling nematodes and developing molecular markers.
The challenges come from compartmentation of metabolites, from dilution of tracer by endogenous pools, and from the difficulty of sampling relevant pools.
The difficulty of sampling airway biochemical markers in humans presents a significant challenge to the evaluation of airway status.
The fauna of the slope and Blake Plateau is poorly known because of the difficulty of sampling deep, hard rocky bottoms under the swift Gulf Stream current.
Due to the high diversity of organisms within the DSL and difficulty in sampling at depth, identifying DSL distribution to species is difficult but relative density offers a valuable snapshot of potential beaked whale prey.
Although indirect evidence suggests the male genital tract as a possible source of persistent HIV shedding in semen during antiretroviral therapy, this phenomenon is poorly understood due to the difficulty of sampling semen-producing organs in HIV+ asymptomatic individuals.
The difficulty of sampling for OTA is most pronounced in the analysis of whole grains.
The anterior superior iliac crest is less preferred due to increased occurrence of pain and difficulty in sampling [ 21].
Because of the difficulty of sampling large facial areas, visible fluorescent tracer was used as the criterion to determine whether the face would be sampled.
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