However, its clinical use is still limited because of technical difficulties and lack of standardisation.
This difficulty in standardisation of imaging tools is partly attributable to the rarity as well as to the heterogeneity of LNETs.
Also, in our study, changes in all variables were related to the individual baseline supine level (by normalisation), reducing the effects of large variations of measured parameters and difficulties with standardisation of the used techniques (TCD, NIRS and BP).
There are also certain difficulties derived from the lack of standardisation in the evaluation of β-catenin expression and the heterogeneity that most colorectal cancers have with respect to the distribution of nuclear β-catenin expression [ 21].
High quality video imaging technology now makes it possible to observe and record the biology of chlamydial infection in detail Technical difficulties of measuring infectivity present problems of standardisation because some strains of C.trachomatis have high particle to infectivity ratios and need to be centrifuged onto host cells to initiate infection [8].
However the main difficulty in entity recognition is the lack of standardisation of the name.
Other limitations include a limited sensitivity during the early phases of illness [ 3], inter-laboratory variation due to subjective interpretation of agglutination and difficulty in standardisation [ 3, 6].
These include patient and surgeon preferences, inability to blind surgeons and difficulties blinding patients, difficulties in obtaining adequate sample sizes, and a lack of standardisation of surgical procedures.
The limitations of this assay are largely related to the difficulty in interpreting the literature due to lack of standardisation of assay reagents, procedures and scoring.
Because of difficulties with standardisation across PET centres and tumour types combined with uncertainty concerning the timing of assessment relative to treatment, the use of quantitative measurements of FDG uptake for evaluating response primarily remains a research tool at present, most applicable to early phase drug trials.
