There will no doubt be recriminations in the coming days - over the lack of shelters, the lack of warning, the difficulties of reconstruction.
The difficulties of reconstruction under both static and movement conditions mainly arise due to muscle properties such as the velocity tension relationship and the length tension relationship.
Note that the number of exons of a gene significantly affects the difficulty of isoform reconstruction.
In addition to reduced length, higher error rates in NGS reads also contribute to the difficulty of genome reconstruction [ 18, 19].
In addition, due to technical difficulties (e.g., incomplete labeling with the Golgi method, the difficulty of 3D reconstruction of the ChC axon coupled with the identification of the postsynaptic target, etc)., meaningful quantitative data are hard to obtain (DeFelipe et al. 1985; Somogyi et al. 1985; Li et al. 1992; Lund and Lewis 1993; Martinez et al. 1996; Krimer and Goldman-Rakic 2001).
In a similar vein, the neatest solution to difficulties of phylogenetic reconstruction may be to focus on experimental systems where the ancestral states are observed directly, as in microbial experimental evolution systems 71 (see below).
Therefore, this landslide cut off the crucial lines of transportation and damaged the public facilities, which increasing the difficulty of rescue and reconstruction after the earthquake to a great degree.
Therefore, the fast landslides triggered by the earthquake have huge destructive power which could easily destroy facilities and structures, and immensely increase the difficulty of rescue and reconstruction after the earthquake.
A second difficulty of tibial prosthetic reconstruction is the considerable biomechanical stress placed on conventional tibial intramedullary stems, which has been associated with relatively high rates of mechanical failure resulting from prosthetic fracture and aseptic loosening.
The difficulty with this view of reconstruction is that it seems to deny the very raison d'être of historical and comparative linguistics.
