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In response to this issue, the manufacturer has made efforts to reduce the difficulty of preparation.
The molecular bases of host cell invasion by the sporozoite of T. gondii are virtually unknown, mostly due to the difficulty of preparation and the transitory nature of this infectious stage, which rapidly differentiates into a tachyzoite inside the host cell.
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They discovered important new facts and solved the great practical difficulties of preparation.
And it is difficult to use a SEM cross section to measure directly the thickness of nano-oxide thin films deposited on the inner surface of glass MCP pores because of difficulty of sample preparation.
Furthermore, pyrazines produced by microorganisms can be found in low quantities in culture media (Beck et al. 2003; Dickschat et al. 2010; Nawrath et al. 2010), and the bottleneck of analysis has been the difficulty of sample preparation due to the high solubility and volatility of pyrazines in microbial samples.
Despite high resolution, lower operation cost and reduced solvent consumption, GC are less commonly used for the analysis of steroids than LC, mainly due to the difficulty of sample preparation, as derivatization should be applied in all studies with GC MS determination [25].
Considering the inherent difficulty of preoperative preparation (i.e., achieving a sufficient fasting period) in pigs, we anticipate that the risk of severe peritoneal contamination could be lower in humans.
This concept might sometimes enhance considerably the difficulty of the dye preparation.
A major limitation of SIT is the difficulty in preparation of effective and safe vaccines from natural allergen sources.
For THA, Janecki and Nelson [ 6] described difficulty in making femoral neck cut, difficulty in preparation of femoral canal and minor intraoperative fracture of lesser trochanter.
The difficulty in preparation of a drug formula that would allow the targeted delivery of NPs to cancer cells is an important factor that restricts the application of photo- and sonosensitizers in cancer therapy.
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