However, it is estimated that due to the difficulties of isolation and culture, a gene pool of 99% of microbial diversity is unknown (4).
To overcome the difficulty of the isolation and to increase the thermodynamic and kinetic stabilities of HSB, various ideas are proposed by making efficient use of the substituent effects - theoretical molecular designing.
Most of these proteins were also identified in the envelope and/or top fractions with high emPAI values, suggesting that these proteins in the nucleoid fractions are contaminated largely owing to the difficulty of nucleoid isolation (see Discussion).
However, the molecular basis determining the differences in biochemical properties of these two MC subclasses remains uncertain, partially due to the difficulty of their isolation.
In an international ballet community with a lack of diversity, she is a rare African-American ballerina, and although she has been shielded from several issues, she endures the difficulties of cultural isolation as the second African-American ABT soloist ballet dancer.
Due to the difficulty in isolation of enough bacteria proteins from infection mixtures and the contamination of host cell proteins, we chose to compare proteomes of BM and BMΔvirB cultured in vitro.
Emotional difficulties, manifested in feelings of isolation and withdrawal from social interaction, were reported by participants in all four groups.
This is mostly due to the difficulty in isolation of somatic stem cells from tissues in significant numbers.
We suspect that development of neutralizing antibody early in symptomatic illness may be the primary factor leading to difficulty in isolation of hantavirus from blood in humans after illness has begun.
