Previous studies have demonstrated the feasibility of engineering brewer's yeast by incorporation of heterologous genes13,28,29; however, the scope and commercial relevance of these efforts have been limited, in part due to methodological difficulties of incorporating an array of large, genetically stable DNA constructs into industrial yeasts.
This is a deliberate choice on our part, due to the difficulty of incorporating the magnitude information, which we consider more than offsets the benefits of the extra information.
One of the problems in modelling solute solvent interactions is the difficulty of incorporating the consideration of solute solvent hydrogen bonding into continuum-type models.
This idea isn't very plausible, but for reasons partly having to do with the problems that Keynesian policymaking was encountering at the time, and partly having to do with the difficulty of incorporating expectations into economic models in any other manner, it proved immensely influential.
Yuan explains that, although microfluidic technology has been extensively developed and widely used for processing small amounts of liquid, it suffers from three inherent limitations related to the devices' overall size, their channel profiles, and the difficulty of incorporating additional materials such as electrodes.
The difficulty of incorporating other minority groups in the area lies in the fact that the historic coexistence of different groups is not based on inclusion, but rather on parallelism.
Owing to the difficulty of incorporating environment-gene with gene-gene interactions, in the present study, we only focused on constructing genetic causal pies for young-onset hypertension.
A further limitation of the Boolean formalism, particularly when focusing on logical steady state analysis with no distinction between processes of different time scales, is the difficulty of incorporating negative feedback loops.
