Sentence examples for difficulty of cultivation from inspiring English sources

Efforts to develop vaccines against these pathogens have been prevented by the difficulty of cultivation of parasites in the laboratory, the complexity of their multicellular organization and many species have been developing impressive antigenic variability.

Here we report the development of a diagnostic DNA microarray for the high-throughput identification of 39 pathogenic bacteria selected based on their high prevalence rate and/or difficulty of cultivation.

Owing to the inherent difficulty of cultivation of T. thermosaccharolyticum DSM 571, it is difficult to obtain a sufficient amount of cells for large-scale enzyme production.

Many medicinal plants, however, are still harvested in the wild due to technical difficulties of cultivation, as well as for economic reasons.

Development of an HCV vaccine has been hampered by the difficulty of in vitro cultivation of the agent and by lack of animal models for studies of viral functions and host immune reactions (1, 2 ).

High exobiopolymer amounts cause high viscosity of the fermentation medium, resulting in slower sugar uptake rate in the latter days of cultivation, difficulty in mixing, and low rate of oxygen transfer.

Dr. Michaels had, after all, grown up on a mushroom farm west of Chicago and had written his thesis on the difficulty of the in-vitro cultivation and growth of T. melanosporum.

Consequently, as a result of the difficulties in outlining a general history of cultivation and domestication, it has been recommended to focus in detail on single crops before thinking about general patterns (Haldorsen et al., 2011; Heun et al., 2012).

Another difficulty is that changes in the pattern of cultivation are usually long-term investments that may be too slow to satisfy immediate pressures for reform.

This stemmed from the difficulty of assessing microbial communities by cultivation methods, which only sampled 0.1%to10%0% of the microbial community [ 4].

Despite their ubiquitousness and apparent significance, our understanding of these microbial communities remains very limited, largely owing to their inherent complexity and the difficulty in laboratory cultivation of most of the microbes.