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The second was the difficulty of assignment of taxonomic groups due to low BLAST scores and similarities (coloured grey in Figure 2).
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This article investigates the difficulty of resonance assignment, using a computational Monte Carlo/simulated annealing (MCSA) algorithm to search for assignments from artificial three-dimensional spectra that are constructed from the reported isotropic 15N and 13C chemical shifts of two proteins whose structures have been determined by solution NMR methods.
This may reflect the greater difficulty of accurate assignment of cause of death as AIDS or non-AIDS in this age group.
Given the (i) multi-domain nature of many protein kinases, (ii) difficulty of domain assignment, and (iii) existence of domain shuffling, we wished to propose a protocol to refine classification that would: take into account full-length sequences, be insensitive to domain detection inaccuracies by avoiding the step of domain detection, take into account domain shuffling.
It also reflected the level of difficulty of the assignment.
Because study participants had a very low dropout rate and complied very well with other aspects of the protocol, it would appear that the poor compliance was due to the difficulty of the assignment rather than to poor motivation.
To define orthologous chromosomal genes among the different L. pneumophila strains, pseudogenes and mobile elements were not taken into account due to the difficulty of ortholog assignment for these genes.
We should differentiate their salaries based on the difficulty of their assignments and their ability to drive results in the classroom.
Major powers expressed regret over Mr. Annan's resignation and acknowledged the difficulties of his assignment, but in doing so they appeared to commit the kind of blame-laying he cited as one reason for quitting.
Boucher said he would wait until Tuesday to consider the difficulty of his next job assignment.
While acknowledging the difficulty of the president's assignment, as well as your own struggles in this arena, it is soothing and clarifying to use history, particularly ancient history, as a reality check.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com