Contrasting with this long-range difficulty of accommodating additional genes, it turns out that gene-specific duplications, which lead to tandemly repeated genes, explain many of the genes found today in the species with the highest number of RBR genes (such as Arabidopsis thaliana or Oryza sativa; Figures 4 and 5).
While a second set of independent samples may be preferable for validation testing, cross validation testing overcomes the recognized difficulty of obtaining additional cohorts.
It follows that genes have a maximum capacity for new isoforms and that once this maximum has been reached, the organizational difficulties of adding additional isoforms will completely outweigh the beneficial effects that these isoforms may provide.
The fact is that for many people already in financial difficulties, any form of additional borrowing (especially one where the repayments relative to income are so high) is liable to make a bad situation worse.
Single SNP differences between related alleles in the non-assayed exons could have potential regulatory or immunologic consequences, although few studies have examined such correlations due to the difficulty of sequencing the additional regions.
Dokmak et al. summarized the features of these substitutes based on six characteristics: availability, difficulty of harvesting, necessity of additional incision, risk of infection, need for long-term anticoagulation, and cost [8].
The privatization of knowledge in the life sciences raises a number of additional difficulties.
Be aware of additional difficulties with concrete subfloors (see Nailing Down the Floor).
But the Predator wasn't designed to be armed – it had difficulty lugging around a lot of additional weight.
The difficulty of obtaining PCR amplification of additional sequences, such as the GroEL and 47-kD protein genes, would be consistent with the identification of a new lineage divergent from Asian forms of O. tsutsugamushi.
