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The D-loop was excluded because of difficulty in alignment.
This large percentage of sites should not be taken to indicate overall difficulty in alignment.
Their usefulness at broader scales (e.g., across the entire family) may be limited by difficulty in alignment and homoplasy.
Loci not represented in both species due to differences in genome coverage, or difficulty in alignment, have not been included.
We also excluded tRNAs associated with the protein-coding sequences, given their short lengths and difficulty in alignment across the large time scales considered here.
However, these genes were removed from further analyses due to difficulty in alignment of these sequences, as such their provenance and evolutionary ancestry remains questionable as noted on figures 2 and 3.
Similar(54)
But given the high evolutionary rate of intron sequence with a lot insertion-deletion (indels), I am suspicious of its feasibility because of the difficulty in alignments to identify the short homologous sequences.
Some families were discarded due to difficulties in alignment or tree reconstruction.
We retrieved plastid gene ycf1 for most taxa (the gene is absent in Sciaphila, see below), but did not include it in the final matrix due to difficulties in alignment.
For analyses including mt CR sequence data, the outgroup taxa were restricted to N. monachus and S. calvus due to difficulties in alignments and presence of indels associated with the other outgroup taxa.
Unfortunately these measurements are associated with some technical difficulties that lead to difficulty in fracture alignment assessment [ 13].
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