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Unfortunately, in today's environment, many early-career scientists have difficulty efficiently transitioning to independent research careers and as a result are choosing paths other than academic research.
We had difficulty efficiently depleting Fra-2 and c-Jun with siRNAs, perhaps because mRNA expression of these genes was relatively low.
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Analyses of Roman mural painting suggest the difficulty in efficiently extracting ancient organic binders.
He says the initiative could help address "a central problem of police forces in the EU: the difficulty to efficiently analyse the images provided by video surveillance.
Besides these factors, often taken into account in such studies [28 34], are two other factors, not usually considered in literature, which may have a decisive influence on results: the difficulty in efficiently extracting organic binders from ancient paint samples, and the presence of organic material due to microbiological contamination.
The two major obstacles hindering the development of an in vitro assay for genome-wide screening are the difficulty of efficiently producing recombinant protein and the inability to detect ubiquitination in a high-throughput fashion.
One of the major challenges in developing a protoplast regeneration system in American elm, as with many other woody species, is the difficulty in efficiently and reproducibly isolating protoplasts [ 15, 16].
Supervisors, maintenance/mechanics, and forklift truck drivers are not eligible due to probable frequent and unpredictable changes in job physical exposures as well as difficulty in efficiently videotaping these workers.
However, many experiments and simulations have shown difficulties in efficiently maintaining sessions' continuity during handover, due to long latency and high packet loss rate issues [2].
You learn to be independent in pursuing research, you become more responsible for your own actions, and you develop the ability to cope with difficulties more efficiently and quickly.
Such studies are not trivial however, because of inherent difficulties in efficiently expressing degenerins using a heterologous expression system.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com