However, although the current study identified substantial areas of difficulty, analysing unprompted annotations has limitations.
ASTRAL had no difficulty analysing these data (completing in <1 s).
Practical problems encountered in getting matched tumour biopsies in all patients were medical contraindication of biopsy, logistic reasons preventing return for a second biopsy and difficulty analysing some of the tissue samples owing to degradation.
Due to difficulties analysing DXR-BMD the relative measure DXR-MCI was used, as described in detail previously [ 6].
An additional difficulty with analyses dependent upon durations of survival is that in the elderly population typical of SQCC, patient deaths not infrequently result from co-morbidities [ 44], such as infection, heart disease, stroke, emphysema and diabetes, rather than from cancer.
The limited number of proteins identified in this study, despite a multi-step approach (enrichment/depletion, SCX, RP-LC-MS/MS), highlights the difficulty of analysing the plasma proteome.
These differences between genders may be due to a greater technical difficulty in analysing female voices [ 56, 60].
Currently, each item has three response options, but the response options 'can carry out' and 'can carry out, but with difficulty' are analysed as one response category.
Our results also suggest a certain difficulty in analysing some variables, which may be derived from a variety of sources, such as the patient or a clinician.
potential differences in adverse events, in particular, those related to gastrointestinal or cardiac difficulties All analyses will be carried out using SAS Version 9.1 (SAS Institute, Cary, NC, USA).
