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Too many yeast cells in a tranformation mix will significantly drop the efficiency and will give difficulties to spread on the plates.
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A comparison of scores, facility, discrimination, reliability and validity from 3-, 4- and 5-option versions of the multiple choice test found little variation in the performance of the three option formats although higher numbers of options tended to increase difficulty and to spread responses between the options.
But it's one thing to talk reasonably about long-term difficulties, and another to spread fear about a bond-default apocalypse.
Other difficulties include Japan's plans to spread the good news about 4G technologies, while the U.S. is still trying to make 3G more widely available.
The decision to target the evolutionary biology section "is sad for the people affected," says Verduyn Lunel, but it's better to concentrate the budget cuts in one area than to spread financial difficulties across all groups.
The more individuals are vaccinated, in this case children, the more difficulties the virus will have to spread in the population.
Nipah case-patients with difficulty breathing were more likely to spread the virus (12% versus 0% P = 0.03) [1].
A McGrath® X blade™ was inserted to spread the larynx; however, difficulties were associated with inserting it into the oral cavity due to trismus.
Despite the relative difficulty of acquiring the disease, it continues to spread.
But despite the difficulties, it is vital that fast, scalable ways to spread excellence are developed.
First, there was the difficulty of synchronising the pseudo-random codes used to spread the signal out at the transmitter, and de-spread it at the receiver.
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