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Despite these difficulties, several groups have reported that GAS6 acts as an acute-phase reactant, increasing its concentration during sepsis [ 8, 9].
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To overcome the difficulty in obtaining tumour samples, several groups have focused on the identification of surrogate markers.
Several groups have reported difficulty in capturing 5′ ends of the primary mRNAs with standard library preparations and directional sequencing strategies [ 14, 27].
Several groups have described these difficulties, the primary one being reliably determining the orthologs among the whole set of homologs detected by exhaustive comparisons between each pair of completely sequenced genomes.
Due to the difficulty of gene expression profiling from FFPET using microarrays, several groups have utilized RT-PCR to quantify the expression of targeted sets of genes [29], [30], [65].
Several groups have announced interest in bidding.
Several groups have been tracking the submissions.
Several groups have already mobilized.
Already this year, several new groups have emerged.
Moreover, several preceding groups had significant difficulty in making the diagnosis.
Due to this difficulty, several species have been grouped into species complexes (reviewed in [28]).
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