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Due to technical difficulties, sampling of the brines was not possible.
The current gold standard for characterizing lesions, histological analysis of tissue biopsies[3], has several disadvantages: expert clinicians are required to collect the samples and interpret results, incurring relatively high costs; inaccuracies in diagnosis due to difficulties sampling tissue which may have multiple dysplastic foci; and patient discomfort with the procedure.
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There are additional challenges with investigating airborne respiratory viruses in critically ill patients due to experimental difficulties in sampling aerosolised virus particles, including the potential inactivation of viruses by current sampling methodology [10].
Still, due to the difficulties of sampling from such a large number of often poorly known species, the assignment of some genera is still awaiting confirmation.
Due to the tight linkage between the additional markers, SimWalk2 had difficulties accurately sampling from the entire sample space.
Challenges arise due to the difficulties of sampling from this site, and the majority of studies have been conducted utilising peripheral blood mononuclear cells.
There are few studies of biological processes during the polar night, largely owing to the logistical difficulties of sampling.
The largest obstacle facing scientists interested in estimating parameters for wild salmonid populations is the difficulty sampling all (or even most) breeding individuals and their offspring, i.e., difficulty obtaining the samples with which to reconstruct the pedigree (e.g., Dickerson et al. 2005).
Nevertheless, their measurement is not easy on account of heterogeneity in property and difficulty of sampling.
However, population genetics studies of nematodes have been constrained due to the difficulty in sampling nematodes and developing molecular markers.
The challenges come from compartmentation of metabolites, from dilution of tracer by endogenous pools, and from the difficulty of sampling relevant pools.
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