The molecular bases of host cell invasion by the sporozoite of T. gondii are virtually unknown, mostly due to the difficulty of preparation and the transitory nature of this infectious stage, which rapidly differentiates into a tachyzoite inside the host cell.
There is no one best budget period or interval the period should fit the needs of a company, in terms of its planning horizon, the difficulties of budget preparation, and the link of the budget to strategic planning.
And it is difficult to use a SEM cross section to measure directly the thickness of nano-oxide thin films deposited on the inner surface of glass MCP pores because of difficulty of sample preparation.
Furthermore, pyrazines produced by microorganisms can be found in low quantities in culture media (Beck et al. 2003; Dickschat et al. 2010; Nawrath et al. 2010), and the bottleneck of analysis has been the difficulty of sample preparation due to the high solubility and volatility of pyrazines in microbial samples.
Despite high resolution, lower operation cost and reduced solvent consumption, GC are less commonly used for the analysis of steroids than LC, mainly due to the difficulty of sample preparation, as derivatization should be applied in all studies with GC MS determination [25].
Considering the inherent difficulty of preoperative preparation (i.e., achieving a sufficient fasting period) in pigs, we anticipate that the risk of severe peritoneal contamination could be lower in humans.
The results demonstrate a feasible approach for addressing one of the potential bottlenecks in inactivated pandemic influenza vaccine production and are particularly important in light of the difficulties in preparation of potency reagent antibody for pandemic H1N1 (2009) virus vaccines.
However, due to the difficulties of the biological preparation as well as real-eye measurements, the theoretical models are considered abstractions and the formulations quite simplistic for the complex mechanism of the eye and vision [ 11, 12].
Allocated drugs were non-blinded owing to the difficulties of arranging six preparations with different dosing schedules, and the impossibility of maintaining blinded allocation with asymmetric timing of insulin therapy initiation.
