However, finding E. coli isolates that co-express LA/AA reiterates the difficulty of assigning bacteria to groups on the basis of their adherence phenotype or genotype (particularly when based on mobile genetic elements).
The difficulties in metabolic engineering of bacteria for terpenoid production normally are not terpenoid detection but problems in the complex metabolic net (Baidoo and Keasling 2013).
However, studies of genetic basis of within-population diversification of Pseudoalteromonas and the ecological consequences of diversification have been very limited, mainly due to the difficulty of genetic manipulation of bacteria within this genus.
It is easy to remember that before microscopes were developed, many people had difficulty believing in the existence of bacteria.
However, the resulting new strains of bacteria still had difficulties growing on DCM.
Historically this has been difficult because of the complexity of the microbiome within oral biofilms, and difficulties in cultivating bacteria obtained from the oral environment.
The anatomical and physiological barriers that the cornea offers against the entrance of bacteria and other pathogens may also pose difficulties for the access and effectiveness of drugs.
Surprisingly, its implementation for bacterial growth inhibition tests is less well reported, perhaps because of the difficulty of fixing/retaining the bacteria on the chip.
These examples indicate the difficulty of inferring a bacteria's exclusivity to an organism based on a lack of its observation in community studies, where only a fraction, albeit an increasing one, of bacterial genera are accessible with current techniques.
However, a complete dissection of Bacillus anthracis is hampered by the difficulty of transforming the bacteria (approximately 10-10 transformants per μg DNA) and by relatively few simple ways to manipulate the genome genetically [ 12- 15].
Such difficulties result in an underutilization of HTS technologies when studying this type of bacteria.
