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However, due to difficulties incorporated with industrial extraction, their commercial use has remained limited.
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To overcome this difficulty, we incorporated tryptic peptides preceding the cleavage sites based on predicted signal sequences from SMART [ 27], SignalP4.0 [ 28], and cleavage sites annotated in HPRD [ 29] into our analysis pipeline.
The use of viral vectors for expression of heterologous proteins in plants is hampered by some limitations including the amount of exogenous genetic information that can be incorporated, difficulties with coexpression of stoichiometric amounts of multiple polypeptides and the risk that infectious clones could escape to environment.
Anisotropic material behaviour is incorporated without difficulties.
The latter incorporated 'some or moderate difficulty' and 'extreme difficulty'.
This is not a technological difficulty, as individuals could be incorporated that have more general schedules.
We used an assessment tool designed for this study to establish the difficulty level of the strategy incorporated into everyday activity.
Pyrosequencing suffers from an inherent difficulty in determining the number of incorporated nucleotides in homopolymer regions, due to the non-linear signal from the incorporation of >5 identical nucleotides [ 14].
Anthropogenic disturbances impact freshwater biota but are rarely incorporated into conservation planning due to the difficulties in quantifying threats.
However, we noted difficulty applying the questions so we developed an enhanced version of the OQAQ which incorporated guidelines for its' use [ 12].
However, Doshi said the experience helped the team understand the difficulties in creating something that's too isolated from the core Mixpanel platform, a lesson they incorporated into the subsequent, successful launch of a product focused on user data.
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