Sentence examples for difficulties in sequencing from inspiring English sources

Polymorphic positions that are not associated with single-nucleotide repeats were selected for validation, because pyrosequencing is known to experience difficulties in sequencing these regions [ 39].

Affected family members carry a heterozygous R553H mutation yielding difficulties in sequencing mouth movements, as well as impaired expressive and receptive language [2].

In 1992, Mori et al. first described the presence of Aβ3 pE) using mass spectrometry of purified Aβ protein from AD brains, explaining the difficulties in sequencing the amino-terminus [ 41].

These data suggest that the reduced intergenic coverage in our nucleosome-bound DNA sequencing data set is not simply the result of technical difficulties in sequencing the highly AT-rich intergenic regions of the P. falciparum genome.

The major drawback using this approach are difficulties in sequencing stretches of identical nucleotides (homopolymeric regions) longer than approximately five nucleotides due to the nonlinear light response they generate [ 10].

However, the extreme repetition within the extender domains, which can be identical even at the DNA level, has caused difficulties in sequencing the ORFs (open reading frames) of some RTX adhesins.

The ambiguous bases in the ENSEMBL genome sequence may result from the difficulty in sequencing the repetitive region, or perhaps from the polymorphic state of the MITE in the sequence template.

Another limitation of the Sanger method is its difficulty in sequencing GC-rich regions.

A few mutations in the sense strand were introduced in all constructs to avoid plasmid instability and difficulty in sequencing.

33 Another difficulty in sequencing Plasmodium clinical samples is posed by polyclonality, that is, mixed genotype infections.

Because of ease of sequence analysis, DArT markers have a significant advantage over AFLPs for positional cloning efforts due to the difficulty in sequencing AFLPs that, therefore, cannot be readily integrated into the whole genome sequence.