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Study of M. genitalium genetic variation has been hampered by the limited availability of genetic materials due to difficulties in isolation and cultivation of this organism from clinical specimens.
However, ethical concerns in using ESCs and difficulties in isolation, expansion, and differentiation of adult stem cells limit their clinical application.
However, cilia proteome, particularly that of 9+0 cilia, is not easily available, largely due to the technical difficulties in isolation of the minute organelle.
Elucidating the physiological significance of an orphan cytochrome P450s is a complicated process hampered by difficulties in isolation and purification of CYPs as well as identifying possible substrates from a myriad of potential chemicals.
Isolation and ex vivo expansion of cardiac endothelial cells have been a recurrent challenge due to difficulties in isolation, cell heterogeneity, lack of specific markers to identify myocardial endothelial cells, and inadequate conditions to maintain long-term cultures.
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This is mostly due to the difficulty in isolation of somatic stem cells from tissues in significant numbers.
Due to the difficulty in isolation of enough bacteria proteins from infection mixtures and the contamination of host cell proteins, we chose to compare proteomes of BM and BMΔvirB cultured in vitro.
We suspect that development of neutralizing antibody early in symptomatic illness may be the primary factor leading to difficulty in isolation of hantavirus from blood in humans after illness has begun.
These utilize a range of techniques from plastic adherence and Percoll gradients to immunodepletion; however, investigators continue to articulate difficulties in the isolation and expansion of mMSCs2,3,4,5.
But as all too many people with severe chronic diseases know, loved ones can disappear, leaving them to bear their difficulties in lonely isolation.
So far, no studies are available in non-CF bronchiectasis patients evaluating difficulties in NTM isolation in case of a P. aeruginosa coinfection.
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