Sentence examples for difficulties expression from inspiring English sources

After removing data related to 3 genes for which there were technical difficulties, expression changes of 39 genes were appropriately measured based on analysis using a linear model (See Methods S1 in Supplementary Online Materials).

(2) Our inducible protein library was undoubtedly only partial due to the Sau3A partial digestion used to generate library clones, the effect of codon bias on protein expression, and difficulties expressing all antigens (especially membrane associated proteins) equivalently in E. coli based expression libraries.

That imperfect fluency allows for reflection on difficulties of expression and communication that are endemic to all of our attempts to make meaning.

Difficulties in expression, purification, and crystallization of these transmembrane receptors are the reasons for the low success rate.

Integral membrane proteins (IMPs) control the flow of information and nutrients across cell membranes, yet IMP mechanistic studies are hindered by difficulties in expression.

Due to difficulties in expression, secretion, and post-translational modification, antibodies intended for clinical use remain a challenge to produce in a cost-effective manner (Buckholz and Gleeson 1991; Huang et al. 2014).

The use of intein-based systems provides a site-selective method but is often limited due to difficulties with expression of large target-intein fusion proteins and side reactions of the thiol based chemistry.

However, confirmatory biochemical IC50 determinations were precluded by difficulties in expression of catalytically active TbAUK1.

Infections and/or painful states set in, but sedative, analgesic and antibiotic medication are not provided due to difficulties of expression and perception in neurodegenerative pathology.

Although, we were unable to purify the protein, due to difficulties of expression in E. coli, activity tests performed on crude extracts, revealed significant differences in the kinetic activity of the two enzymes.

Patient exclusions were Glasgow Coma Scale verbal component rated as none or sounds only, moderate-severe dysphasia (grossly impaired comprehension, unintelligible speech, or major difficulties in expression), non-English speaking, learning disability, major deafness or blind, or readmission of patient previously included in the study.