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While the NS culture model has its limitations, the use of acutely isolated cortical tissue has its own limitations, specifically that the tissue itself will be a heterogeneous mix of cells (including postmitotic cells) and it is difficult to precisely ascertain the proliferation and potential of this heterogeneous population of cells.
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Currently, it is difficult to precisely clarify the excitation mechanism.
It is therefore difficult to precisely identify all compounds [ 78].
It is difficult to precisely define repetitive regions in a genome.
The complex milieu of in vivo amyloid pathology is difficult to precisely reproduce in experimental models.
It is very difficult to precisely quantify the extent of use of contraband cigarettes.
In other words, it is difficult to precisely time-lock the moment of "getting" a joke.
Although some progress has been made [ 11- 14], it is still difficult to precisely identify combinatorial motifs.
It was difficult to precisely evaluate the beginning of the outbreak.
It is difficult to ascertain precisely what the hundreds of nongovernment groups in Haiti spent on their response to the earthquake — at least $1.5 billion, probably much more — and how they spent it.
The prevalence of capital punishment in ancient times is difficult to ascertain precisely, but it seems likely that it was often avoided, sometimes by the alternative of banishment and sometimes by payment of compensation.
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CEO of Professional Science Editing for Scientists @ prosciediting.com