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Loans for development remain difficult to obtain, construction is slow and vacancy rates continue to climb.
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Mr. Barnett said in an interview that, with construction financing for large projects still difficult to obtain, he believes his building is years ahead of anything comparable entering the market.
However, the proximity of the gtl-1 and gtl-2 loci (13 kbp) makes it very difficult to obtain the double mutant by standard strain construction methods.
The pellet was washed with 0.7 M NaCl for three times, with 1.2 M sorbitol once and then resuspended in a minimal volume (usually ~1 ml) of 1.2 M sorbitol to reach a compromise to obtain both as high as DNA concentration and as many as DNA plugs required for at least one attempt of BAC library construction (It is difficult to obtain a high DNA concentration from rice fungi).
But, it is difficult to control these factors individually, optimized structure is also difficult to obtain.
Palestinian construction is normally allowed here, but permits are very difficult to obtain.
In the construction of these strains, we noticed that it was difficult to obtain diploids if both parental cells were of the pom152 ∆ mps3 ∆ genotype.
Mr. Rosenfeld said that while it might be difficult to obtain financing for the redevelopment at present, the downturn was a perfect time to begin obtaining the approvals needed to begin construction.
Details are difficult to obtain.
Some are extremely difficult to obtain.
Clear information proved, in the end, difficult to obtain.
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