But that advice should come with an important caveat: once those e-mail messages start arriving, it can be difficult to know a bargain from a tease: that hotel room advertised for less than a night at the movies could end up costing as much as a monthly car payment once you click through to actually book it.
"It's difficult to know, difficult to know," a Yahoo employee said when asked about the matter.
The Per2 reporter we use is longer and contains E-boxes, D-boxes, and ROR elements, making interpretation of its direction difficult to know a priori.
Considering the alternative, where the investigator uses the minimum number of loci due to time or financial constraints, it would be difficult to know a priori which loci in combination yield trees with the most resolution and highest node support.
Illumina protocols recommend 10 cycles of PCR when starting with one microgram of input DNA, and 18 cycles when starting with 5 nanograms, but it is difficult to know a priori how to optimize cycle number for alternative sample amounts.
Without cloning, it is difficult to know a priori which reads came from which transcripts; and many less highly or less broadly expressed transcripts are only weakly or incompletely supported by current RNA-Seq.
The leaking electrons make it more difficult to know when a transistor is in an on or off state, the information that makes electronic computing possible.
But many European artisans also settled in England and trained locals, making it difficult to know whether an object was made by foreigners or natives.
It is difficult to know when a person's ethnicity makes a difference and mediates a person's relationship with healthcare services as there are many other factors such as socioeconomic status, age, education which come into play [ 19].
