It is difficult to amplify fragments of DNA more than 300 base pairs from old samples [4], [6].
It is, however, difficult to infer the fragment for large insertions directly from the read sequence.
Open reduction and internal fixation (ORIF) is often difficult to achieve, as fragment displacement, comminution and osteoporotic bone quality act as limiting factors.
Conversely, it is more difficult to find small fragments with high individuation-specific information than to find intermediate-sized fragments with low amounts of relevant information.
By including a subtractive amplification, Me-RDA and HMe-RDA can enrich rare fragments or sequences difficult to amplify because of fragment size or base content that would not have been enriched using a single ligation-mediated HELP PCR.
However, due to the relative protein load of a total skin homogenate, it is difficult to observe these fragments on Western blot.
As this long fragment was difficult to obtain in tumor samples, a shorter fragment of ECRG4 was amplified using forward primer ECRG4_RT_for2: 5'-GGTACCAGCAGTTTCTCTACATG-3' and the reverse primer stated above at a Ta of 62°C (220 bp).
Jumping libraries are more difficult to create, and 'small' fragment libraries (up to 500 bp on the HiSeq and 600 bp on the MiSeq instrument) are the fastest, most efficient way to generate deep coverage of a genome today.
We find it difficult to understand how a fragment of LL-37 would inhibit membrane interactions, and favour the hypothesis that LL-37 interacts with ERBB kinases via a yet unidentified receptor.
This difference is mainly due to the fact that it is more difficult to find intermediate-sized fragments with small detection-specific information than it is to find small fragments with high task-specific information.
Overlapping fragments were initially difficult to differentiate on ultrasound as the fragments gave the impression of alignment when viewed in a perpendicular plane.
