Moreover, conserved regions with ICS are difficult to align well by one single alignment.
PG in general handles better these difficult to align datasets by combining a local alignment strategy with a more flexible way of assigning motif sites.
Some sequences, particularly ribosomal genes such as 18S, are known to be difficult to align properly [ 98], and these alignment ambiguities can significantly affect phylogeny reconstruction [ 95, 99].
First, the genome alignment considers contiguous sequence similarity, which makes it difficult to align some local regions.
Traditional internal alignment accuracy decreases as evolutionary distance increases; different sequences are difficult to align accurately.
These repeats typically generate regions in the alignment with high variability in sequence and length, and that are difficult to align.
But I found it difficult to align my personal principles to my professional life until one conversation helped me to clarify things.
Simulation also verifies that it is quite difficult to align the interference perfectly with the AHT algorithm.
The previous apparatuses of femtosecond pulse shaping are 4f Fourier-transforming type system that is usually large, expensive, difficult to align.
However, at present, the analysis of next-generation sequencing data is challenging, in particular because most sequencing platforms provide short reads, which are difficult to align and assemble.
Similar to small tumors, LN PET images are difficult to align perfectly with CT images that often have poor soft tissue contrast.
