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Anterior cruciate ligament (ACL) is one of the most difficult tissues to heal once injured.
This result may reflect the difficulty in diagnosis: FA and N maintain microscopic follicular structures; FA and FC are the most difficult tissues to be differentially diagnosed.
Both the spleen and the placenta were the most difficult tissues to perfuse, as their open circulation proved difficult for the complete removal of blood.
TUNEL assays were done using the In Situ Cell Death Detection kit (Roche) according to the supplier's protocol for difficult tissues.
In situ cell death detection kit, TMR red (Roche version April 2006) was used to stain apoptotic cells following the manufacturer's protocol for difficult tissues except that goat serum was used in the blocking step instead of bovine serum.
Therefore, customizable materials that can achieve such mechanical regimes and persist for long periods of time in culture should be of interest for functional cell studies which mimic these difficult tissues.
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Skin is a difficult tissue for automated image analysis due to its heterogeneous composition and its architecture.
Lungs are the most difficult tissue.
Similarly, polypeptide ligands that bind to cartilage are designed for delivery into this difficult tissue (US Patent 7592009).
However, challenges exist with cancer biomarkers, including unsatisfactory specificity and sensitivity, difficult tissue accessibility, and ineffective test for detection.
Spleens are frequently considered a "difficult" tissue due to their relatively high abundance of nucleases [ 26] which makes the RNA present in spleen tissue more prone to degradation.
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