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And the flesh-eating reputation is a difficult stain to lose.
Furthermore, the thermostability feature of B. stratosphericus lipase is an added value as high temperatures are often preferable to remove difficult stain in cotton and synthetic materials (Bora and Bora 2012).
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Finally, in Phase 3, the role of Gordon Sweet's Silver staining was determined for the assessment of a third set of cases, to see if this laborious and difficult staining procedure is even necessary.
The absence of detectable root-colonization by Acaulospora could be due to incompatibility with common beans, or difficult staining of the root-colonization structures [ 34], or that staining of Acaulospora root colonization is tricky, it may be a false negative, since some Acaulosporas are difficult to stain with the blue dye [ 35].
Some difficult stains require more than one treatment.
Oils and salts in your perspiration may damage the paper and leave difficult stains.
You can use a power washer to remove difficult stains from exterior concrete.
Remove all difficult stains with a piece of bamboo or a wooden spatula.
Clean off wine, tomato sauce or other difficult stains with a paste of stone cleaner and baking powder.
Difficult stains can be treated with ammonia at source by dabbing them with a sponge, which has been dipped in ammonia.
The problem is that the tissue is extremely unstable and difficult to stain, yielding very messy sections, and we see staining for a variety of markers making the results so far rather equivocal for understanding the pathway of cell death.
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