Unfortunately, in recent years, many hybrid algorithms have been very complex, so these complex algorithms are not applicable or are very difficult in applications.
Thus, most linkage maps with important trait loci have been developed with primarily low-throughput, anonymous, dominant markers such as RFLPs, RAPDs or AFLPs [ 28- 31], making them difficult in applications like map-based cloning, and maker-assisted selection.
However, in this study, we did not investigate how sensitive the model simulation results are to the cut off threshold values, therefore, further studies need to investigate how the threshold value should be chosen in order to provide stabilization (may be difficult) in the application of the GLUE method.
Its hardware implementation is difficult in practical applications.
Although these methods can map the distorted water surface, they appear to be difficult in practical applications in hydraulic engineering laboratories.
As Bai [5] pointed out, it is a difficult task in applications of autoregressive models.
One of the most difficult problems in applications of semi-parametric partially linear single-index models (PLSIM) is the choice of pilot estimators and complexity parameters which may result in radically different estimators.
(3) Pixel calculation Because it is difficult to segment the image accurately, it is difficult, in the practical application, to count the number of pixels of the part touching the image boundary and the total number of pixels of the entire object.
However, those materials exhibited a high preparation cost and low stability in the process, being difficult their application in an industrial wastewater treatment plant.
This goal is specially more difficult in real-time applications in outdoor scenes, which present a challenge due to the variant illumination and atmospheric conditions such as clear, hazy, or cloudy days.
