Many methods used to remediate AMD are limited in implementation due to poor performance, design inaccuracies, difficult understanding of functionality, high costs, usage of hazardous chemicals, depletion of natural resources and the generation of further waste.
If the mutation happened during evolution, inferred from comparisons of mutant with a more ancestral outgroup, it is even more difficult to predict functionality.
Yet, since the joint contribution of multiple 'doses' is likely to determine transcriptional outcomes, it may be difficult to infer functionality of a given TFBS in isolation from others.
First, the short lengths of the identified ORFs and their general lack of detectable homology or reading-frame conservation with related species make it difficult to infer functionality [ 37, 38].
However the large number of asRNAs detected makes it difficult to unravel functionality and physiological impacts of antisense transcription, and this paper should therefore be read as a documentation of the potential asRNA repertoire from which specific studies can be designed.
"As soon as you release an app into the wild it's difficult to control the functionality of it," says Top10.com co-founder Harry Jones.
It's not as simple as drag-and-drop, and without Apple's proprietary TuneKit library, some functionality is difficult or impossible to replicate at this point.
Other existing energy system models that do not have this emphasis on compactness and openness makes the barrier to entry by new users much higher, as well as making the addition of innovative new functionality very difficult.
Assuring the smooth interoperability of all software components is sometimes difficult, as is balancing functionality with simplicity for sites with minimal infrastructure.
This lack of conservation, coupled with conflicting evidence and hypotheses for VirB6 channel structure formation [89], [90] (Figure 3B), makes it difficult to predict the functionality of any of the five duplicate VirB6-like proteins in Rickettsia.
