The work was dangerous because the rock at the Horsforth end was difficult to blast, and there was frequent flooding and subsidence.
In particular, sequence families exhibiting only short conserved blocks interspersed with highly variable regions are difficult for BLAST or BLAT[ 3] because the seeds have to be very short in this case.
Given the inherent potential for misassembly or chimeric transcripts in de novo assembly, it is difficult without strong BLAST support to present the additional putative teretoxins identified via Pepticomb as high confidence transcripts.
They are unaffected by the presence of water, which had long made blasting difficult for open-pit miners.
This makes application of tools like PSI-BLAST difficult, since it relies on clear conservation patterns.
The royal commission has opened and sanctioned a hunting season on Bill Shorten, notwithstanding his structural security as federal Labor leader, courtesy of that Rudd caucus rule that makes it extremely difficult for colleagues to blast him out of his job.
Included in this selection are: implosions in planar, cylindrical, and spherical coordinates, piston problems with a variety of mesh configurations including the difficult Saltzman problem, interacting blast waves, and a spherical expansion on a rectangular mesh.
Mα genes are often difficult to identify by Blast alone because their sequence evolves rapidly.
It is therefore unlikely that the failure to classify these contigs as cnidarian or dinoflagellate was simply attributable to their being shorter than average and thus more difficult to annotate by BLAST or to align to genomic reads.
Exons smaller than 20 codons are difficult to identify by BLAST, so if a pillar that generates a hit contains a small exon, only the larger exon(s) are usually detected in a TBLASTN search, and therefore the hit is flagged for manual annotation.
