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Although as old as that of chemical procedures, enzyme-mediated peptide ligation is far less developed than that of chemical counterpart due to the difficult availability of peptide ligase.
The lower amount of cumulative mineralized carbon at 30 °C may be accounted for by the faster depletion of labile carbon during the prior incubation stage and the difficult availability of recalcitrant carbon at the late incubation stage.
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It provides useful information of suspicious lesions, increasing the visibility of malignant lesions, in particular in women with dense breasts, and can be an alternative to contrast-enhanced MRI, especially in the case of contraindications to MRI or to gadolinium-based contrast injection as well as of difficult MRI availability.
Software is now both central and essential to modern biology, yet lack of availability, difficult installations, and complex user interfaces make software hard to obtain and use.
Anyhow, with minimally invasive methods, the accuracy in the diagnosis of sinus membrane perforation is difficult without the availability of endoscope.
However, isolation of bonafide regulatory T-cells remains difficult because the availability of specific marker molecules is still limited.
In addition, most studies have been conducted in batch mode, preventing separation between growth and enzyme production and making carbon source availability difficult to control [ 26, 37].
Counting the actual number of loci has been notoriously difficult without the availability of genome sequences, and rough estimates of copy number for highly multiplicated MHC I genes have been obtained from blotting experiments [ 62, 63].
Moreover, poor availability, difficult access, and reluctance to utilize oral health services, even those publicly funded, may be significant barriers to the adoption of oral health behaviors that prevent disease.
leading to a bio-distribution and bio-availability difficult to analyze in details without a widespread investigation that is beyond the scope of this work.
Attaining high plant availability is difficult because the fusion power core has a limited service lifetime.
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