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By comparing the cytosolic, membrane, and nuclear fractions of the two groups, 178 significantly (P < 0.05, ANOVA) differing spots were found, corresponding to 139 unique proteins.
Figures 1– 3 indicate the positions of significantly differing spots of the cytosolic, membrane and membrane organelle, and nuclear subproteomes, respectively.
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However, differing spot positions will still occur in any nontrivial experiment that includes more than one gel.
Coordinates of significantly differing protein spots (p≤0.05 with FDR correction, r a t i o≥|3| when comparing both treatments) were transferred to a pick list for further processing.
To understand how the Islamic State economy functions is to delve into a murky world of middlemen and shady business dealings, in which "loyal ideologues" on differing sides spot business opportunities and pounce upon them.
A student's t-test was used to compare mean densities of protein spots, and spots differing by 2-fold expression or greater were selected.
In some cases, the same immunoreactive protein focused to more than one area on a gel, with protein spots differing slightly in isoelectric point.
In addition to the gel-to-gel variations, these normalization methods have to cope with differing backgrounds and differing distributions of spot quantities between dyes.
The other two of the 11 proteins were detected as pairs of spots differing in both molecular weight and isoelectric point (SCO5465, SCO2271).
Quantitative spot analysis of the young and senescent cell proteome revealed 29 spots differing significantly in abundance (p ≤ 0.05)).
For example, the membrane protein FTT_0583 was observed to be immunoreactive in two discrete protein spots, differing in both MW and pI (Figure 1a & b).
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