One of these differences between the chromosomes of Bbsl species is attributable to differing sequences at the origin of replication in the middle of the chromosome.
The most likely reason why these primers did not amplify the other 13 E. moshkovskii DNA samples is that they differed in sequence in the primer-binding regions.
We used the MatInspector tool of the Genomatix suite which uses position-specific weight matrices (PWMs) to calculate matrix similarity scores for the two versions of the sequence differing only in the SNP nucleotide.
In the cystine rich RING-type zinc finger domain, the sequences differ in the spacing between the second and third cystine.
While all known p1 alleles share the P1-rr promoter and proximal enhancer sequences, they differ in the sequence arrangement that contains the distal P1-rr enhancer.
Three preparation methods, differing in the sequence of ultrasonic treatment application, were applied to prepare PP/CNT composites and investigate the bubble cavitation mechanism inside and outside of CNT agglomerates in composites.
In addition, the amino acid sequences differ significantly in the stretch (CDSSLS, drd1; CIIAN-, drd2; Figure 1) between the disulfide bridge and TM5, which may be crucial contributors to the D2/D1 receptor agonist selectivity.
B3-544, B3-544, B3-601 and B3-607 were allelic variants, sharing over 99% of identity and their deduced protein sequences differed only in the repetitive domain with the deletion of glutamine residues or one repetitive unit (Additional file 1: Figure S4).
Then we asked whether the observed nucleotide sequence differs from the random sequences in the frequency of short nucleotide repeats, and thus in terms of stability We again first focused on mononucleotide repeats, and found that these repeats are strongly under-represented in coding regions of M. tuberculosis.
These sequences only differ in the nucleotides at the terminal end of the ligation product, suggesting that these are 5' mismatch ligations of the Jurkat sequence.
