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To further investigate the relationship between DNA hypomethylation, radiosensitivity and genomic stability in stem-like cells we have studied mouse embryonic stem cells containing differing levels of DNA methylation due to the presence or absence of DNA methyltransferases.
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Post-gastrulation tissue-specific epigenetic modification can also occur at MEs, resulting in differing absolute levels of DNA methylation between the tissues of one individual, but the persistence of the similar magnitude of interindividual methylation variation when the same tissues are compared between different organisms.
Indeed, our work clearly shows that whereas the luminal-B and luminal-A subtypes do not differ in terms of the specific FEMs (since for both subtypes, the FEM deviation scores are higher compared to normal tissue), they do however differ significantly in terms of the absolute levels of DNA methylation and gene expression deviation from the normal reference.
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