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Three successive differentiations of each line were quantified (Fig. 3e), each grow beginning as 75 cm2 original surface area of subconfluent hESC.
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Furthermore, the fabricated SERS-active Au nanodots modified surface was used to differentiate the biochemical compositions of single cells at different spots, which represent the differences in biochemical compositions, enabled the differentiation of each state and the conditions of cells.
In both cases, the maximum order of differentiation of each output appearing in the model inversion constitutes relevant information since it characterizes the regularity of the trajectory to be followed.
Elemental analysis (such as scanning electron microscopy with energy dispersive analysis or X-ray fluorescence) could be used as a complementary technique [29], but the differentiation of each type by the presence of silica could be misleading because of the frequent combination with earth pigments that contain quartz [30].
The differentiation of each single population by FiSH showed, as in the metaphase assay, that the relationship between viable maternal and neonatal cells was shifted significantly (p<0.05) towards the neonatal cells, while the relationship of the viable cells in the MLCs between unrelated adults was balanced.
The overall differentiation of each population was estimated as the mean pairwise FST between each population and all others.
The enhanced Raman signals achieved from each cell, which represent the changes in biochemical compositions, enabled differentiation of each state and the conditions of the cells.
Several genes involved in thyroid development (most of which encode homeodomain proteins) have been identified, the molecular mechanisms that regulate the development and differentiation of each type of thyroid cells remains unknown.
The TF Brn4 was included as a 'downstream' acting TF to maintain symmetry between the lineages so as to ensure the same time scale for differentiation of each the cell types (several days for each).
Notwithstanding, these data suggest that a large proportion of protein domains were invented in the root or after the separation of the three major superkingdoms but before the further differentiation of each lineage.
The levels of each marker increased substantially during differentiation of each cell line.
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